Use of Response Surface Methodology to Predict Optimal Conditions of Kluyveromyces lactis Permeabilization by a Physical Method
نویسندگان
چکیده
J. Teles de Faria, M. Lopes Moraes, A. Del Borghi, A. Converti, F. M. Lopes Passos, L. A. Minim, A. P. de F. C. Vanzela, and F. Coelho Sampaio Department of Food Technology, Federal University of Viçosa, Av. P. H. Rolfs s/n, 36571-000 Viçosa, Minas Gerais, Brazil Department of Pharmacy, Federal University of Jequitinhonha and Mucuri, Rua da Glória, 187, 39100-000 Diamantina, Minas Gerais, Brazil Department of Chemical and Process Engineering, University of Genoa, via Opera Pia 15, 16145 Genoa, Italy Department of Microbiology, Federal University of Viçosa, Av. P. H. Rolfs s/n, 36571-000 Viçosa, Minas Gerais, Brazil
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Statistical investigation of Kluyveromyces lactis cells permeabilization with ethanol by response surface methodology
The aim of our study was to select the optimal operating conditions to permeabilize Kluyveromyces lactis cells using ethanol as a solvent as an alternative to cell disruption and extraction. Cell permeabilization was carried out by a non-mechanical method consisting of chemical treatment with ethanol, and the results were expressed as β-galactosidase activity. Experiments were conducted under d...
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